Sertraline, Piperine and Sorafenib Combinations Attenuate Liver Cancer Progression: Genotoxic and Oxidative stress Effects
DOI:
https://doi.org/10.5281/zenodo.21500030Keywords:
Genotoxicity, Hepatocellular carcinoma, Oxidative stress, Piperine, Sorafenib, Sertraline.Abstract
Objective: This study aimed to estimate the effects of the combination of sorafenib (SOR), piperine (PIP), and sertraline (SER) on oxidative stress and DNA damage in hepatocellular carcinoma (HepG2) cells.
Materials-Methods: The MTT technique was employed to determine the effect of the SOR+PIP+SER combination on cytotoxicity, the comet assay was used to determine its effects on genotoxicity, and the Total antioxidant (TAS) oxidant levels (TOS) assay was used to determine its effects on oxidative stress.
Results: The results showed that, following combination treatment, the low (SOR2.5+PİP7.5+SER0.62) and medium-dose (SOR5+PİP15+SER1.24) combination groups reduced SOR-induced DNA damage, while SOR further increased DNA damage levels. It was determined that PIP and SER administered at different doses along with SOR in the experimental groups increased oxidative stress levels compared to the control group.
Conclusion: As a result, it was determined that combination therapy may have anticarcinogenic effects, particularly at high doses, due to its genotoxic and cytotoxic effects on HepG2 cells.
Keywords: Genotoxicity, Hepatocellular carcinoma, Oxidative Stress, Piperine, Sorafenib, Sertraline.
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