Assessment of Prolidase, MDA, Catalase and GSH Levels İn Hemorhoid Patients
DOI:
https://doi.org/10.5281/zenodo.19297939Keywords:
Antioxidant, Oxidative stress, Reduced glutathione, HemorrhoidsAbstract
Objective: Many methods are used in the surgical treatment of hemorrhoidal disease in today's World. The purpose of this study; The aim of this study is to examine prolidase, MDA, catalase and GSH parameters among 30 patients between the ages of 18-65 diagnosed with hemorrhoids and a group of 30 healthy volunteers between the ages of 18-65, and to investigate the relationship between the two groups.
Materials and methods: In this study, two groups were formed: a patient group of 30 individuals aged 18-65 with grade 1 internal hemorrhoids and no other diseases, and a healthy control group of 30 individuals aged 18-65 without any disease. The activities of the parameters were measured spectrophotometrically in this study.
Results: The difference between the mean values of the patient and control groups was found to be statistically significant (p<0.05). Accordingly, GSH and CAT were low, while prolidase and MDA were high in the patient group.
Conclusion: Based on these data, it can be concluded that oxidative stress negatively affects hemorrhoid disease and that antioxidants may have a positive effect in the treatment of hemorrhoid disease.
Keywords: Antioxidant, Hemorrhoids, Oxidative stress, Reduced glutathione
References
REFERENCES
[1].Ekçi, B., Tanrıöver, Ö., İzbırak, G., & Gökçe, Ö. (2009). Birinci basamakta hemoroid hastalığına pratik yaklaşım. Türk Aile Hekimleri Dergisi, 13(2), 87-92.
[2].Rakinic, J., & Poola, V.P. (2014). Hemorrhoids and fistulas: new solution the old problems. Current Problems of Surgery, 51(3), 98-137.
[3].Erdoğdu, A. (2011). Hemoroidektomi uygulanan hastaların postoperatif hayat kalitesi değişikliklerinin incelenmesi (Uzmanlık tezi). İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, İstanbul.
[4].Panahi, Y., Mousavi-Nayeeni, M. S., Sahebkar, A., Fanaie, A. S., Rahimnia, A., & Beiraghda, F. (2014). Hemorrhoids: A randomized double-blind, double-dummy parallel-group comparative study. Turkish Journal of Pharmaceutical Sciences, 11(1), 1–8.
[5].Salinitri, P. D., & Raspallo, P. M. (Eds.). (2010). Hemorrhoids. Nova Science Publishers.
[6].Buğra, D. (2012). Açık Hemoroidektomi. Türkiye Klinikleri Dergisi, 5(1), 63-67.
[7].Geçim, E.İ. (2011). Hemoroidal Hastalık. Kolon Rektum Hastalıkları Dergisi, 21, 145-159.
[8].Teksöz, S. (2008). Hemoroidektomilerde damar kapama sistemi (Ligasure) ile konvansiyonel Ferguson yönteminin karşılaştırılması: Prospektif randomize klinik çalışma (Uzmanlık tezi). İstanbul Üniversitesi Cerrahpaşa Tıp Fakültesi, İstanbul.
[9].Zheng, J. S., Huang, T., Li, K., Chen, Y., Xie, H., Xu, D., Li, D. (2015). Modulation of the association between the PEPD variant and the risk of type 2 diabetes by n-3 fatty acids in Chinese Hans. Lifestyle Genomics, 8(1), 36–43.
[10].Pandit, R. A., Chen, C. J., Butt, T. A., & Islam, N. (2013). Identification and analysis of a novel mutation in the PEPD gene in two Kashmiri siblings with prolidase enzyme deficiency. Gene, 516(2), 316–319.
[11]. Farmer, E. E., & Mueller, M. J. (2013). ROS-mediated lipid peroxidation and RES-activated signaling. Annual Review of Plant Biology, 64, 429–450.
[12].Uchida, K. (2000). Role of reactive aldehyde in cardiovascular diseases. Free Radical Biology and Medicine, 28(12), 1685-1696.
[13].del Rio, D., Stewart, A. J., & Pellegrini, N. (2005). A review of recent studies on malondialdehyde as a toxic molecule and biological marker of oxidative stress. Nutrition, Metabolism and Cardiovascular Diseases, 15(4), 316–328.
[14].Draper, H. H., & Hadley, M. (1990). Malondialdehyde determination as an index of lipid peroxidation. Methods in Enzymology, 186, 421–431.
[15].De Duve, C. (1983). Microbodies in the living cells. Scientific American, 248(1), 42–52.
[16]. Masters, C., & Holmes, R. (1977). Peroxisomes: New aspects of cell physiology and biochemistry. Physiological Reviews, 57(3), 866–882.
[17].Gülçin, I. (2002). Isırgan otunun (urtica dioica) antioksidant aktivitesinin belirlenmesi, oksidatif enzimlerin karakterizasyonu ve bazı ın vivo etkilerinin incelenmesi, (Doktora Tezi), Atatürk Üniversitesi, Fen Bilimleri Enstitüsü, Erzurum.
[18].Demir, H., Alkan, S., Savran, A. (2004). Sığır karaciğerinden saflaştırılan katalaz enzimi üzerinde bazı ilaçların inhibisyon kinetiğinin incelenmesi. XVIII. Ulusal Kimya Kongresi, Kars
[19].Mates, J. M., Pérez-Gómez, C., & Núñez de Castro, I. (1999). Antioxidant enzymes and human diseases. Clinical Biochemistry, 32(8), 595–603.
[20].Alving, A., Carson, P., Flanagan, C. L., & Ickes, C. (1956). Enzymatic deficiency in primaquine-sensitive erythrocytes. Science, 124(3214), 484–485.
[21].Halliwell, B., & Gutteridge, J. M. C. (1989). Free radicals in biology and medicine (2nd ed.). Clarendon Press.
[22].Fridovich, I. (1999). Fundamental aspects of reactive oxygen species. Annals of the New York Academy of Sciences, 893, 13–18.
[23].Halliwell, B. (1999). Antioxidant defence mechanisms. Free Radical Research, 31, 261–272.
[24].Kirkman, H. N., Rolfo, M., Ferraris, A. M., & Gaetani, G. F. (1999). Mechanisms of protection of catalase by NADPH. Journal of Biological Chemistry, 274, 13908–13914.
[25].Terblanche, S. E. (2000). The effects of exhaustive exercise on catalase activity. Cell Biology International, 23, 749–753.
[26].Dringen, R. (2000). Metabolism and functions of glutathione in brain. Progress in Neurobiology, 62, 649–671.
[27].Cnubben, N. H. P., Rietjens, I. M. C. M., Wortelboer, H., Van Zanden, J., & Van Bladeren, P. J. (2001). The interplay of glutathione-related processes. Environmental Toxicology and Pharmacology, 10, 141–152.
[28].Terpstra, M., Henry, P. G., & Gruetter, R. (2003). Measurement of reduced glutathione in human brain. Magnetic Resonance in Medicine, 50, 19–23.
[29]. Van Bladeren, P. J. (2000). Glutathione conjugation. Chemico-Biological Interactions, 129, 61–76.
[30].Holmgren, A. (2000). Antioxidant function of thioredoxin. Antioxidants & Redox Signaling, 2, 811–820.
[31].Kavas, G. E. (1989). Serbest radikaller ve organizma üzerine etkileri. Türkiye Klinikleri, 9, 1–7.
[32]. Nordberg, J., & Arner, E. S. J. (2001). Reactive oxygen species and the thioredoxin system. Free Radical Biology and Medicine, 31, 1287–1312.
[33].Myara, I., Charpentier, C., & Lemonnier, A. (1982). Prolidase assay. Clinica Chimica Acta, 125(2), 193–205.
[34].Chinard, F. P. (1952). Photometric estimation of proline. Journal of Biological Chemistry, 199(1), 91–95.
[35].Yoshioka, T., Kawada, K., Shimada, T., & Mori, M. (1979). Lipid peroxidation in maternal and cord blood and protective mechanism against activated-oxygen toxicity in the blood. American Journal of Obstetrics and Gynecology, 135(3), 372–376.
[36].Beutler, E., Duron, O., & Kelly, B.M. (1963). Improved method for the determination of blood glutathione. The Journal of Laboratory and Clinical Medicin, 61, 882–888.
[37].Aebi, H. (1984). Catalase in vitro. Methods in Enzymology, 105, 121–126.
[38]. Riss, S., Weisert, F.A., Riss, T., Schwameis, K., Mittlböck, M., & Stif, A. (2010). Hemorrhoids and quality of life. Colorectal Disease, 13(4), 48-52.
[39].Garg, P.K, Kumar, G., Jain, K.B., & Mohanty, D. (2013). Quality of life after stapled hemorrhoidopexy: A prospective observational study. Biomedical Research International Journal, 1-4.
[40].Alparslan, S., Gultepe, M. (1993). Serum prolidase activity: its value as an indicator of collagen accumulation in chronic liver diseases. Turkish Journal of Biochemistry, 18, 1–9.
[41].Ramasamy, I. (2014). Lipoprotein metabolism. Clinical Chemistry and Laboratory Medicine, 52(12), 1695–1727.
[42].Anderson, T. J., Mancini, G. B., Genest, J., Jr., Grégoire, J., Lonn, E. M., & Hegele, R. A. (2013). The new dyslipidemia guidelines: What is the debate? Canadian Journal of Cardiology, 31(5), 605–612.
[43].Phang, J. M., Liu, W., & Zabirnyk, O. (2010). Proline metabolism and microenvironmental stress. Annual Review of Nutrition, 30, 441–463.
[44].Wiegman, A., Gidding, S. S., Watts, G. F., Chapman, M. J., Ginsberg, H. N., Cuchel, M., ... Wiklund, O. (2015). Familial hypercholesterolaemia in children and adolescents: Gaining decades of life by optimizing detection and treatment. European Heart Journal, 36(36), 2425–2437.
[45].Ercan, A. C., Bahceci, B., Polat, S., Cenker, O. C., Bahceci, I., Koroglu, A., & Sahin, K. (2017). Oxidative status and prolidase activities in generalized anxiety disorder. Asian Journal of Psychiatry, 25, 118–122.
[46].Güneş, M., Bulut, M., Demir, S., İbiloğlu, A. O., Kaya, M. C., Atlı, A., Kaplan, İ., Camkurt, M. A., & Sir, A. (2016). Diagnostic performance of increased prolidase activity in schizophrenia. Neuroscience Letters, 613, 36–40.
[47].Kokacya, M. H., Bahceci, B., Bahceci, I., Dilek, A. R., & Dokuyucu, R. (2014). Prolidase activity and oxidative stress in patients with major depressive disorder. Psychiatria Danubina, 26(4), 314–318.
[48]. Liu, C., & Xiao, P. (1992). Recent advances on ginseng research in China. Journal of Ethnopharmacology, 36(1), 27–38.
[49].Marzatico, F., Pansarasa, O., & Bertorelli, L. (1997). Blood free radical antioxidant enzymes and lipid peroxides following long-distance and lactacidemic performances. Journal of Sports Medicine and Physical Fitness, 37, 235–239.
[50]. Sahlin, K., Ekberg, K., & Cizinsky, S. (1991). Changes in plasma hypoxanthine and free radical markers during exercise in man. Acta Physiologica Scandinavica, 142, 275–281.
[51].Lovlin, R., Cottle, W., Pyke, I., Kavanagh, M., & Belcastro, A. N. (1987). Are indices of free radical damage related to exercise intensity? European Journal of Applied Physiology, 56, 313–316.
[52].Mohsin, N. Y. (2020). Determination of oxidative stress level (malondialdehyde), antioxidant activities and ischemia modified albumin in gout patients (Yüksek lisans tezi). Van Yüzüncü Yıl Üniversitesi, Fen Bilimleri Enstitüsü, Van.
[53].Powers, S. K., Ji, L. L., & Leeuwenburgh, C. (1999). Exercise training-induced alterations in skeletal muscle antioxidant capacity: A brief review. Medicine & Science in Sports & Exercise, 31, 987–997.
[54].Gohil, K., Viguie, C., Stanley, W. C., Brooks, G. A., & Packer, L. (1988). Blood glutathione oxidation during human exercise. Journal of Applied Physiology, 64, 115.
[55]. İnal, M., Akyüz, F., & Turgut, A. (2001). Effect of aerobic and anaerobic metabolism on free radical generation in swimmers. Medicine & Science in Sports & Exercise, 33(4), 564–567.
[56].Koyu, A., Özgüner, F., Çalışkan, S., & Karaca, H. (2005). Preventive effect of vitamin E on iron-induced oxidative damage in rabbit. Toxicology and Industrial Health, 21(7), 239-242.
[57].Yıldırım, I., Kılınç, M., Okur, E., Tolun, F. İ., Kılıç, M. A., Kurutaş, E. B., & Ekerbiçer, H. Ç. (2007). The effects of noise on hearing and oxidative stress in textile workers. Industrial Health, 45(6), 743–749.
[58].Storey, B. T. (1997). Lipid peroxidation and its role in sperm function and dysfunction. Molecular Human Reproduction, 3(3), 203–213.
[59].Agarwal, A., & Allamaneni, S. S. R. (2004). Role of free radicals in female reproductive diseases and assisted reproduction. Reproductive BioMedicine Online, 9(3), 338–347.
[60].Kassab-Chekir, A., Laradi, S., Ferchichi, S., Khelil, A. H., Feki, M., Amri, F., ... & Miled, A. (2003). Oxidant, antioxidant status and metabolic data in patients with beta-thalassemia. Clinica Chimica Acta, 338(1-2), 79-86.
[61].Valente, F., & Waletzky, J. A. (1994). Determination of catalase activity in biological samples. European Journal of Clinical Chemistry and Clinical Biochemistry, 32(8), 583–587.
[62].Michiels, C., Raes, M., Toussaint, O., & Remacle, J. (1994). Importance of antioxidant enzymes for cell survival. Free Radical Biology and Medicine, 17(3), 235–248.
[63].Mezzetti, A., Lapenna, D., Pierdomenico, S. D., et al. (1995). Plasma antioxidants and markers of oxidative stress in atherosclerosis. Atherosclerosis, 112(1), 91–106.
[64].Vertuani, S., Bosco, E., Testoni, M., Ascanelli, S., Azzena, G., & Manfredini, S. (2004). Antioxidant herbal supplements for hemorrhoids: Developing a new formula. Nutrafoods, 3(3), 19–26.
[65].Young, I.S., & Woodside, J.V. (2001). Antioxidants in health and disease. Journal of Clinical Pathology, 54(3), 176–186.
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